Cell culture is the process used to keep cells alive in the laboratory. The process has undergone many changes since its discovery in 1907, as better techniques, equipment, and reagents continue to be developed. Cell culture now relies on a toolkit of techniques (e.g. aseptic technique), equipment (e.g. biosafety cabinet), and reagents (e.g. flasks and growth medium). Cells can be maintained and grown from many species and tissues, ranging from humans to fish, and from skin to brain. New methods and uses are emerging, allowing scientists to grow stem cells and develop more effective models through three-dimensional culture. However, cell culture can be challenging. Problems can affect any cell culture practitioner, even the most seasoned expert. Training is needed to ensure aseptic conditions, skilled handling, and an awareness of safety issues and of the purity and identity of the material being handled. New in the 8th edition 1. A new chapter will examine the origin and evolution of cell cultures and cell lines. This will consist of existing content that will be moved from elsewhere, and new content on stem cell behavior and induced pluripotent stem cells. 2. A new chapter will discuss irreproducible research, its causes, and the importance of good cell culture practice. Previous editions have included a great deal of information on these topics. Moving existing content to a dedicated chapter will give a stronger focus on reproducibility and good cell culture practice. 3. An existing chapter will be modified to provide a new focus on gene delivery and gene editing, with particular emphasis on CRISPR/Cas9.  4. Updates will be made to techniques and applications throughout the book, including live cell imaging, genotyping, stem cell culture, three-dimensional culture, microfluidics, high-throughput screening, and toxicity testing. 5. For the 7th edition, some specialized protocols were removed from the print copy and made available through the books companion website. This will continue for the 8th edition. Protocols will be prepared in individual files using a print-ready format, making it easier to upload and generate links for the electronic version of the book.