This systematically designed laboratory handbook elucidates a number of techniques which help the students carry out various experiments in the field of biochemistry. The experimental protocols described in this book have been standardized and performed in the author’s own laboratory. It is basically intended for the undergraduate and postgraduate students of life sciences (biochemistry, microbiology, biotechnology, plant biotechnology, animal biotechnology, botany and zoology) and engineering (biotechnology and biomedical) for their laboratory courses. The students usually have to refer to many journals and books to find the right procedure for performing experiments, hence this handbook is an attempt to provide them with the frequently used methods in a handy format, including explanations of principles, procedures and interpretations of results of the experiments. Now, in its second edition, the book introduces ten new experiments in a step-by-step procedural format under In Vitro Enzymatic Anti-oxidant Assays explaining Determination of Nitric Oxide Radical Scavenging Activity, Determination of Catalase Activity, Determination of Laccase Activity and Concentration and Diafiltration. KEY FEATURES Provides a general procedure of the experiments in an easy-to-understand tabulated format. Presents the physiological importance of bio-components like amino acids, uric acid, carbohydrates, proteins, etc. in the human body as an added feature. Gives information on preparation of laboratory reagents in separate appendices. Provides illustrations for better understanding of the experiments. This book is primarily intended for the first year B.Tech students of all branches for their course on engineering chemistry. The main objective of this book is to provide a broad understanding of the chemical concepts, theories and principles of Engineering Chemistry in a clear and concise manner, so that even an average student can grasp the intricacies of the subject. It includes the general concepts of structure and bonding, phase rule, solid state, reaction kinetics and catalysis, electrochemistry, chemical thermodynamics and free energy. Besides, the book introduces topics of applied chemistry like water technology, polymer chemistry and nanotechnology. Each theoretical concept is well supported by illustrative examples. The book also provides a large number of solved problems and illustrations to reinforce the theoretical understanding of concepts. KEY FEATURES Each chapter of the book provides a clear and easy understanding of the definitions, theories and principles. A large number of well-labelled diagrams help to understand the concepts easily and clearly. Chapter-wise glossary and important mathematical relations are given for quick revision. Provides multiple choice questions with answers, short questions and long questions for practice.