Written for bioscientists working with microbial, plant and animal cells and tissues, this instructive guide enables its readers to master all aspects of scanning electron microscopy and successfully apply it to their object of study. Starting with an introduction to the principles of electron microscopy and the operation of an electron microscope, it goes on to describe the preparation and mounting of specimens, the process of image taking and their subsequent analysis, including advanced techniques such as cryo-SEM, tomography, FIB-SEM and stereo-SEM. More than 700 illustrations, including hundreds of microscopic images, provide hands-on practical advice on how to perform a successful TEM experiment, with the advantages and challenges of the different TEM modes clearly spelled out.