The book brings together all important principles of nonlinear optical (NLO) microscopy. By presenting NLO microscopy within a consistent foundational framework, the origin of the signals and the interrelation between different NLO techniques can be understood. The book provides rigorous yet practical derivations, which amount to expressions that can be directly related to measured values of resolution, imaging speed, sensitivity and imaging contrast. The book addresses typical questions users and students ask and answers them with clear explanations and examples. Readers of this book will develop a solid physical understanding of NLO microscopy, appreciate the advantages and limitations of each technique, and recognize the exciting possibilities that lie ahead.

Eric Olaf Potma is Professor at the University of California, Irvine, in the Department of Chemistry. His research interests are quantitative imaging with nonlinear optical microscopy, nonlinear optics of individual molecules and nanostructures, and nonlinear optical scan probe microscopy.